Polymorphism of LRP4 Gene (rs9667108) among Post Menopause Women with Osteoporosis
| Autor: | Saeid Kavoosian, Alimohammad Asgharian, Mohsen Asouri, Sadegh Fattahi, Galia Amirbozorgi, Ali Kheirandish, Sajad Abolfazli, Maryam Lotfi, Nima Ebrahimi, Ramin Ataee |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Iranian Journal of Public Health. |
| ISSN: | 2251-6093 2251-6085 |
| DOI: | 10.18502/ijph.v52i4.12457 |
| Popis: | Background: Many studies have been done to identify the factors that influence the development and progression of osteoporosis. One genetic factor is polymorphisms of LRP4 gene. Regarding the lack of comprehensive study on polymorphisms of LRP4 gene in the north of Iran, mainly Mazandaran Province, we decided to investigate the polymorphism of this gene in postmenopausal women with osteoporosis. Methods: This case-control study has been conducted at GhaemShahr Valiasr Hospital on 100 female patients with osteoporosis (average age of 58.1) and 90 healthy females without osteoporosis (average age of 55.2). After sampling and extraction of genomic DNA via of the salt deposition method, the genotype and SNP (rs9667108) polymorphism of LRP4 gene were evaluated with the PCR-RFLP method. Restriction enzymes cut the PCR products. In order to identify patients, their bone mineral density was tested by the DEXA method .The results of digestion (digestion enzyme) were analyzed by MedCalc, SPSS software, Hardy-Weinberg equilibrium, and Chi2. Results: The statistical analysis has shown the significant relationship between SNP (rs9667108) polymorphism and the risk of osteoporosis disease in patients and control groups (P |
| Databáze: | OpenAIRE |
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