A 70-kDa apolipoprotein designated ApoJ is a marker for subclasses of human plasma high density lipoproteins
| Autor: | William D. Stuart, H. V. De Silva, C. R. Duvic, J. R. Wetterau, M. J. Ray, Judith A.K. Harmony, H. W. Albers, W. R. Smith, Donald G. Ferguson |
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| Rok vydání: | 1990 |
| Předmět: |
Gel electrophoresis
chemistry.chemical_classification Apolipoprotein B biology Cholesterol Cell Biology Biochemistry chemistry.chemical_compound High-density lipoprotein chemistry Affinity chromatography Cholesterylester transfer protein biology.protein lipids (amino acids peptides and proteins) Centrifugation Glycoprotein Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 265:13240-13247 |
| ISSN: | 0021-9258 |
| Popis: | A new apolipoprotein, termed apolipoprotein J (apoJ), was purified from human plasma by immunoaffinity chromatography. ApoJ is a glycoprotein consisting of disulfide-linked subunits of 34-36 and 36-39 kDa. Each subunit is glycosylated and has a pI range of 4.9-5.4. ApoJ exists in the plasma associated with high density lipoproteins (HDL) and specifically with subclasses of HDL which also contain apoAI and cholesteryl ester transfer protein activity. Immunoaffinity purified apoJ-HDL subclasses have apparent molecular masses of 80, 160, 240, 340, and 520 kDa, as determined by gradient gel electrophoresis. By negative staining electron microscopy, apoJ-HDL range in diameter from 5 to 16 nm. Fractionation of plasma by vertical gradient density centrifugation revealed apoJ-HDL in HDL2 (d 1.063-1.125 g/ml) with the majority overlapping HDL3 (d 1.125-1.21 g/ml) and very high density lipoprotein (d 1.21-1.25 g/ml). The bimodal density distribution of apoJ-HDL suggests that these subclasses have a unique metabolic relationship and may play a role in the transport of cholesterol from peripheral tissues to the liver. |
| Databáze: | OpenAIRE |
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