A Randomized Controlled Trial of Peripheral Blood Mononuclear Cell Depletion in Experimental Human Lung Inflammation
| Autor: | William A Wallace, Alison Fletcher, Chiara McCormack, Niall Anderson, Timothy S. Walsh, Nikhil Hirani, Mairi Brittan, Daniel F. McAuley, Laura C. Barr, Andrew Conway Morris, Kevin Dhaliwal, Dilip Patel, Marc Turner, Donald J. Davidson, Hamish Richardson, Martin Connell, Adriano G. Rossi, Lynn Manson, A. John Simpson |
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| Rok vydání: | 2013 |
| Předmět: |
Pulmonary and Respiratory Medicine
Lung medicine.diagnostic_test Inhalation business.industry Inflammation Leukapheresis Critical Care and Intensive Care Medicine Peripheral blood mononuclear cell Neutrophilia Bronchoalveolar lavage medicine.anatomical_structure Immunology Clinical endpoint Medicine medicine.symptom business |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 188:449-455 |
| ISSN: | 1535-4970 1073-449X |
| DOI: | 10.1164/rccm.201212-2334oc |
| Popis: | Rationale: Depletion of monocytes reduces LPS-induced lung inflammation in mice, suggesting monocytes as potential therapeutic targets in acute lung injury.Objectives: To investigate whether depletion of circulating blood monocytes has beneficial effects on markers of systemic and pulmonary inflammation in a human model of acute lung inflammation.Methods: A total of 30 healthy volunteers were enrolled in a randomized controlled trial. Volunteers inhaled LPS at baseline, and were randomized to receive active mononuclear cell depletion by leukapheresis, or sham leukapheresis, in a double-blind fashion (15 volunteers per group). Serial blood counts were measured, bronchoalveolar lavage (BAL) was performed at 9 hours, and [18F]fluorodeoxyglucose positron emission tomography at 24 hours. The primary endpoint was the increment in circulating neutrophils at 8 hours.Measurements and Main Results: As expected, inhalation of LPS induced neutrophilia and an up-regulation of inflammatory mediators in the blood and lu... |
| Databáze: | OpenAIRE |
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