Metabolism of Thiabendazole in Laying Hen and Lactating Goats
| Autor: | William J. A. VandenHeuvel, Peter G. Wislocki, Timothy D. J. Halls, Amechi C. Chukwudebe, Dale R. Sanson |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Benzimidazole Metabolite General Chemistry Urine Metabolism Biology High-performance liquid chromatography chemistry.chemical_compound Endocrinology Sulfation Animal science chemistry Internal medicine medicine Anthelmintic General Agricultural and Biological Sciences Feces medicine.drug |
| Zdroj: | Journal of Agricultural and Food Chemistry. 42:2964-2969 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Thiabendazole (TBZ), an anthelminthic and fungicide of the benzimidazole class, was rapidly metabolized by lactating goats and laying hens. In lactating goats dosed orally with 120 mg of [14C]TBZ daily for 7 consecutive days and sacrificed at about 24 h after the final dose, an average of 74% of the total administered dose was accounted for in the matrices analyzed. Nearly all of this recovered radioactivity was found in the excreta (urine, 69%; feces, 28%) with very little (less than or equal to 3%) in milk and tissues (liver, kidney, fat, muscle). In laying hens dosed orally with 3.19 mg of [14C]-TBZ daily for 10 consecutive days and sacrificed at about 24 h after the final dose, an average of about 97% of the total administered dose was accounted for in the matrices analyzed. Nearly all (>99%) of this recovered radioactivity was found in the excreta with very little in the eggs (approximately 0.16%) and tissues (0.29%). Enzyme preparations (Glusulase, sulfatase, beta-glucuronidase) and strong acid hydrolyses were used to release TBZ and its metabolites from hen and goat tissues and matrices. On the basis of these enzymic and acid hydrolyses, and subsequent HPLC radiochromatographic and GC/MS analyses of the extracts, the major metabolite of TBZ in the excreta, edible tissues, milk, and eggs was determined to be 5-hydroxythiabendazole or its O-sulfate conjugate. Minor amounts of benzimidazole and TBZ were also found in most of the goat and hen tissues examined. The results of this study show that the metabolic fates of TBZ in lactating goats and laying hens are similar, predominantly involving hydroxylation at the 5-position followed by sulfation. |
| Databáze: | OpenAIRE |
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