Stability of Trichoderma harzianumamdS transformants in soil and rhizosphere

Autor:	Z. Barak, Oded Yarden, I. Chet, S. Pe'er
Rok vydání:	1991
Předmět:	Rhizosphere education.field_of_study Inoculation Population food and beverages Soil Science Trichoderma harzianum Fungi imperfecti Biology biology.organism_classification complex mixtures Microbiology Trichoderma Soil water Botany education Selectable marker
Zdroj:	Soil Biology and Biochemistry. 23:1043-1046
ISSN:	0038-0717
DOI:	10.1016/0038-0717(91)90041-h
Popis:	Trichoderma harzianum was transformed with a plasmid p3SR2, carrying the dominant selectable marker amdS. The amdS transformants can be identified, from non-sterile soil. on Trichoderma-selective medium (TSM). containing acrylamide as the sole nitrogen source. The transformants were stable after at least 6 weeks in non-sterile soil in the greenhouse and after 14 months in sterile soil at 4°C. Soil inoculation resulted in effective colonization of the roots by the transformants. The advantages of these transformant fungi in the study of fungal ecology and population dynamics is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::841fa1213ba1fae9faea50f05b787268 https://doi.org/10.1016/0038-0717(91)90041-h Zobrazit plný text záznamu