Interplay of DNA and RNA N6-methyladenine with R-loops in regulating gene transcription in Arabidopsis
| Autor: | Jingjing Gao, Wenli Zhang, Pengyue Zhang, Yilong Feng, Xinxu Li, Yining Shi, Manli Shi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology RNA Plant Science Biology biology.organism_classification 01 natural sciences Cell biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Histone chemistry Transcription (biology) Arabidopsis biology.protein Arabidopsis thaliana Molecular Biology Gene DNA Function (biology) 010606 plant biology & botany |
| Zdroj: | Physiology and Molecular Biology of Plants. 27:1163-1171 |
| ISSN: | 0974-0430 0971-5894 |
| Popis: | R-loops and covalent modifications of N6-methyladenine on DNA (D-6 mA) or RNA (R-m6A) have been documented to function in various cellular processes in eukaryotes. However, the relationships between R-loops and both covalent modifications are still elusive in plants. Here, we integrated existing ssDRIP-seq with D-6 mA and R-m6A data from Arabidopsis thaliana. We found that the presence of either of both modifications facilitates R-loop formation and transcription of overlapping genes. Interestingly, our study suggests that the presence of R-m6A is key to affect R-loop intensity and positively regulate gene transcription. Moreover, the presence of D-6 mA plays an additive role to facilitate the effect of R-m6A on R-loop intensity, however, D-6 mA may negatively regulate gene transcription when coexisted with R-m6A. Our analyses indicate that D-6 mA, R-m6A, or histone marks may act individually and cooperatively with R-loops in regulating gene transcription. Our study is the first to link R-loops with D-6 mA and R-m6A in plants, thereby providing new insights into interactions between R-loops with D-6 mA, R-m6A, and histone marks for regulating gene transcription. |
| Databáze: | OpenAIRE |
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