Optical Sol-Gel Materials Based on Binding and Catalysis by Biomolecules

Autor: Joan Selverstone Valentine, Bruce Dunn, Stacey A. Yamanaka, K. E. Chung, Esther H. Lan, Jeffrey I. Zink
Rok vydání: 1994
Předmět:
Zdroj: MRS Proceedings. 346
ISSN: 1946-4274
0272-9172
DOI: 10.1557/proc-346-1017
Popis: The proteins copper-zinc Superoxide dismutase (CuZnSOD), cytochrome c, myoglobin, hemoglobin, and bacterio-rhodopsin are encapsulated in stable, optically transparent, porous, silica glass matrices prepared by the sol-gel method such that the biomolecules retain their characteristic reactivities and spectroscopic properties. The resulting glasses allow transport of small molecules into and out of the glasses at reasonable rates but retain the protein molecules within their pores. The transparency of the glasses enables the chemical reactions of the immobilized proteins to be monitored by means of changes in their visible absorption spectra. Silica glasses containing the immobilized proteins have similar reactivities and spectroscopic properties to those found for the proteins in solution. The enzymes glucose oxidase and peroxidase were also encapsulated in transparent silica glass matrices. Upon exposure to glucose solutions, a colored glass is formed that can be used as the active element in a solid state optically based glucose sensor. Likewise, gels containing oxalate oxidase and peroxidase exhibit spectroscopic changes upon exposure to aqueous solutions containing oxalic acid.
Databáze: OpenAIRE
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