Immunohistochemical study of cardiolipin and phosphatidylinositol in mouse liver
| Autor: | Beloshapkina Td, Khramkova Ni, Iakimenko Ef, Gleĭberman As |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
biology
medicine.diagnostic_test Phospholipid General Medicine Immunosorbents Immunofluorescence Molecular biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound chemistry Biochemistry biology.protein Cardiolipin medicine Immunohistochemistry lipids (amino acids peptides and proteins) Phosphatidylinositol Antibody Hapten |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 86:1206-1209 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/bf00845030 |
| Popis: | The localization of phospholipid haptens (cardiolipin and phosphatidylinositol) in frozen and paraffin sections of mouse liver fixed in acetone and in an acetone-buffer-formalin mixture was studied by the indirect fluorescent antibodies method. Antiphospholipid sera specifically stained the plasma membranes of the hepatocytes, especially the region of the membrane facing the blood sinus. Detection of phospholipid haptens in liver sections with the aid of antiphospholipid sera depends on the method of obtaining and fixing the sections. Depending on the method of immunization, two types of antiphospholipid sera are obtained; they differ in their stability, in the possibility of isolating antibodies on lipid immunosorbents from them, and in their ability to stain liver sections. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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