Detection of Candida Albicans in some enteropathy patients using PCR technique
| Autor: | Ahmed Adnan Abbas, Haider Mahmooed Jasim, Ali Hasanain Alhamadani |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
food.ingredient
biology Pcr cloning Early detection biology.organism_classification medicine.disease Isolation (microbiology) Corpus albicans Microbiology law.invention food law medicine Agar Enteropathy Candida albicans Pharmacology Toxicology and Pharmaceutics (miscellaneous) Polymerase chain reaction |
| Zdroj: | International Journal of Pharmaceutical Quality Assurance. 10 |
| ISSN: | 0975-9506 |
| DOI: | 10.25258/ijpqa.10.3.23 |
| Popis: | Laboratory identification based on DNA amplification methods by used PCR may provide an alternative and more sensitive method than traditional culture for the early detection of Candida albicans. This study aims to the identification and determines the prevalence of C. albicans by use PCR method. A total of 167 samples (40 controls and 127 patients) were taken .By the use of culture techniques for isolation and identification C. albicans on Sabouraud dextrose agar are characterized by cream to white and smooth at 30C◦ these characteristics. C. albicans have been isolated from patients' samples and these included the C.albicans was detected in 17 control subjects (42.5%), and in 51 patients (40.16%). The results of this estimation revealed that the amplified DNA (PCR product) was 459bp for C.albicans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|