Production of Live Piglets Following Cryopreservation of Embryos Derived from In Vitro-Matured Oocytes1

Autor:	Hiroshi Nagashima, Hitomi Matsunari, Ryo Tomii, Naoki Nakayama, Mayuko Kurome, H. Saito, K. Hiruma, S. Ueno
Rok vydání:	2007
Předmět:	Fetus animal structures In vitro fertilisation medicine.medical_treatment Embryo Embryo culture Cell Biology General Medicine Parthenogenesis Anatomy Biology Cryopreservation Andrology Reproductive Medicine Embryo cryopreservation embryonic structures medicine Vitrification
Zdroj:	Biology of Reproduction. 76:900-905
ISSN:	1529-7268 0006-3363
Popis:	We have successfully produced healthy piglets following cryopreservation of embryos derived from oocytes matured and fertilized in vitro. The appropriate timing of cryopreservation pretreatment (removal of cytoplasmic lipid droplets [delipation] and vitrification) was initially determined using parthenogenetic embryos derived from in vitro-matured (IVM) oocytes. Viable embryos were obtained at the highest rate when embryos were delipated at the four- to eight-cell stages (Day 2 of embryo culture) and were vitrified approximately 15 h later (Day 3) by means of the minimum volume cooling method. After cryopreservation of embryos derived from oocytes matured and fertilized in vitro under the most appropriate conditions, 401 embryos were transferred to five recipient gilts, and the recipients all became pregnant. At autopsy of one of the recipients, which had received 47 embryos, eight fetuses (17.0%) were found. Three recipients each gave birth to two to four piglets (1.4%–6.0%). These results demon...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::83de73586ff7d0f1fed92f614cfe619a https://doi.org/10.1095/biolreprod.106.052779 Zobrazit plný text záznamu