| Autor: |
Karl H. Norris, Sam Asen, R. N. Stewart |
| Rok vydání: |
1975 |
| Předmět: |
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| Zdroj: |
Phytochemistry. 14:937-942 |
| ISSN: |
0031-9422 |
| DOI: |
10.1016/0031-9422(75)85162-4 |
| Popis: |
A microspectrophotometric method has been developed for calorimetric pH determination using indicator dyes. Tissue samples as small as five cells were used. Measurements of standard buffered solutions of known pH were within a standard error of less than ± 0.04 pH units. Validity of the technique has previously been established by matching the pH values and absorption spectra of several model systems to that of living cells. A method for spectrophotometric pH determination of single cells is suggested. The pH change seemed to be the major factor in the color change in aging flowers. The epidermal pH, the absorption spectra of living tissue, the anthocyanidins, flavonols, and flavones present in more than 250 plants of many families were determined. These data indicate that pH is only one of numerous parameters determining flavonoid color in the living cell. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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