| Autor: |
Miwa Fujiwara, Rene Patino, Gary Kukes, Jeffery Lee, Catalina Guerra, Catherine Sassoon, Chetty Kota, Farhad Mazdisnian |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
A68. TISSUE REMODELING IN THE LUNG. |
| DOI: |
10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a2129 |
| Popis: |
RATIONALE: The reported incidence of tracheobronchomalacia(TBM) in patients undergoing broncoscopy is 12-44%.Teflon has been successfully used in Vocal cord dysphonias to stiffen the paralyzed cord.We evaluated the effect of Teflon injection on histopathology of pig’s trachea with the intention to assess whether Teflon injection induces fibrosis for potential treatment of TBM. METHODS: Seven Yucatan Pigs were studied.Six animals were assigned to the treatment arm,and one to the control arm.The duration of study was twelve weeks.Under general anesthesia animals were intubated.0.3-0.5ml of sterile Teflon paste in 50% glycerin was injected into the Posterior tracheal wall.The control pig received an equivalent amount of Glycerin.One animal was euthanized weekly from weeks 1-4,8 and 12.After euthanasia,trachea was immediately excised and placed in formalin solution.The tissue was stained with H&E. RSSULTS: Tissue reaction was seen in all specimens from the experimental arm.Most prominent reaction was observed after 4 weeks of Teflon injection.The tracheal wall showed an intense fibro-histiocytic and chronic inflammatory reaction to teflon particles.This resulted in collogen production by fibroblasts as thin pink bands running horizontally across.The control tracheal wall showed only a few red blood cells outside small vessels and iron containing hemosiderin granules in macrophages.In all sections,the cartilage was normal and there was no granulation inside the trachea.C ONCLUSIONS: TBM can produce dynamic outflow obstruction with symptoms of dyspnea, and intractable cough.Treatment is complicated with significant morbidity and mortality.The proposed study may shed light into an alternative method in treatment of TBM. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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