Development of Proplastids and Accumulation of LHCP II Apo-Protein in Golgi and Thylakoids of Dark-Grown Wax-Rich Cells of Euglena Gracilis at Low Light Intensities seen by Immuno-Electron Microscopy
| Autor: | Tetsuaki Osafune, Jerome A. Schiff, Tomoko Ehara, Shuji Sumida, Eiji Hase |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Chlorophyll b
Euglena gracilis biology Chemistry ved/biology Immunoelectron microscopy ved/biology.organism_classification_rank.species food and beverages Immunogold labelling Golgi apparatus biology.organism_classification Euglena Chloroplast symbols.namesake chemistry.chemical_compound Thylakoid Botany polycyclic compounds symbols Biophysics lipids (amino acids peptides and proteins) |
| Zdroj: | Regulation of Chloroplast Biogenesis ISBN: 9781461364856 |
| DOI: | 10.1007/978-1-4615-3366-5_50 |
| Popis: | When dark-grown resting cells of Euglena are exposed to light at the low intensity threshold of chloroplast development(7 ft-c) the antennas, including the LHCP II 26.5 kD apoprotein are very low or undetectable, but on exposure of these cells to more normal intensities of light for development (80–150 ft-c) the antennas, including the LHCP II apoprotein and chlorophyll b rapidly accumulate 1. Thin sections of similar cells at 3–7 ft-c do not show appreciable staining of either Golgi or thylakoids with rabbit immune serum against the 26.5 kD apoprotein plus protein A-gold, but when these cells are incubated at 80–150 ft-c specific staining with immunogold rapidly appears in both the Golgi and the thylakoids 2,3. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|