Effect of ascorbic acid and hemin on experimental colon carcinogenesis in mice
| Autor: | Eun-Young Kim, Jae-Hwang Jeong, Sang Yoon Nam, Lee， Beom-jun, Ye Seo Jeong |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Journal of Biomedical and Translational Research. 21:177-183 |
| ISSN: | 2508-139X 2508-1357 |
| DOI: | 10.12729/jbtr.2020.21.4.177 |
| Popis: | Colon cancer is known as the third most widespread cancer in the world. The interaction of heme-iron and ascorbic acid (AA) in colon carcinogenesis is not evident. Hemin (ferric chloride heme) is an iron-containing porphyrin with chlorine that can be formed from a heme group. The purpose of this study was to investigate the protective effect of AA on the formation of pre-neoplastic lesions induced by azoxymethane (AOM)/dextran sodium sulfate (DSS) plus hemin in mice. Forty-five ICR male mice were divided into three experimental groups; AOM/ DSS treatment (control group), hemin (2 g hemin/kg of b.w.), hemin + AA (1.0% in drinking water). The mice had three s.c. injections (0–2nd weeks of the experiment) of AOM (10 mg/kg b.w.) weekly and 2% DSS as drinking water for the next one week and the animals fed on AIN-76A purified rodent diet for 6 weeks. The numbers of aberrant crypt foci (ACF) and aberrant crypts (ACs) in colonic mucosa were counted after methylene blue staining. Lipid peroxidation in feces was measured by the thiobarbituric acid-reactive substances (TBARS) assay. The numbers of ACF and ACs per colon significantly increased in Hemin group compared to the control group. However, the numbers of ACF and ACs per colon notably decreased in hemin + AA group compared to the control group or hemin group (p |
| Databáze: | OpenAIRE |
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