Cell surface expression and alloantigenic function of a human class I MHC heavy chain gene (HLA-B7) in transgenic mice
| Autor: | J W Chamberlain, J A Nolan, S H Gromkowski, K A Kelley, J M Eisenstadt, K Herrup, C A Janeway, S M Weissman |
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| Rok vydání: | 1988 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 140:1285-1292 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.140.4.1285 |
| Popis: | We have introduced the gene encoding the heavy chain of the human MHC class I Ag HLA-B7 into transgenic mice. The gene was shown to be expressed at both the RNA and protein level. Cell surface HLA-B7 was detected on whole spleen cells by immunoprecipitation and on purified T cells by flow cytometry (FACS). Normal mice immunized with H-2-syngeneic B7-transgenic spleen cells generated CTL capable of killing transgenic cells and B7-expressing human JY cells. Anti-HLA mAb blocked the killing of JY cells. These results indicate that the human class I Ag HLA-B7 can be expressed at the surface of transgenic spleen cells in the absence of human beta 2-microglobulin, and that a significant fraction exists in a form recognizable by nontransgenic CTL as a major histocompatibility Ag unrestricted by H-2. |
| Databáze: | OpenAIRE |
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