Hyper-production of manganese peroxidase by mutantPleurotus ostreatusMTCC 142 and its applications in biodegradation of textile azo dyes
| Autor: | Shahul Hameed Sheik Abdulla, Mani Arunkumar |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
biology
Bran Chemistry digestive oral and skin physiology Ion chromatography food and beverages Ocean Engineering Biodegradation biology.organism_classification Pollution chemistry.chemical_compound Sodium nitrate Manganese peroxidase Botany Glycerol Ammonium Pleurotus ostreatus Water Science and Technology Nuclear chemistry |
| Zdroj: | Desalination and Water Treatment. 56:509-520 |
| ISSN: | 1944-3986 1944-3994 |
| DOI: | 10.1080/19443994.2014.937766 |
| Popis: | Enhanced production of manganese peroxidase (MnP) by the mutant Pleurotus ostreatus MTCC 142 was carried out in a solid and submerged fermentation by using an agro-industrial waste (rice bran and wheat bran) under optimized conditions. Among the various substrate combinations examined, rice bran shows the highest MnP activity (273 U/L), when compared with the wheat bran (218 U/L). Maximum MnP (368 U/L) was produced when rice bran (5 g) at 75% moisture (w/v) was used with 1 mL inoculum at pH 4 and 25°C in the presence of 2% (w/v) glucose as a carbon source, 0.2% (w/v) sodium nitrate as a nitrogen source and 4% of (v/v) glycerol as an inducer for MnP production under submerged fermentation. MnP was purified from the culture fluid by using microfiltration (1.2 μm) and ammonium sulphate precipitation followed by ion exchange chromatography. Additionally, the protein content of the recovered supernatant was also noted. The purified MnP was a monomer, which showed a molecular mass of 58 ± kDa as determi... |
| Databáze: | OpenAIRE |
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