Expression of gene for N-acyl-homoserine lactonase AiiA affects properties of rhizospheric strain Pseudomonas chlororaphis 449

Autor:	E. E. Atamova, Inessa A. Khmel, V. A. Lipasova, M. A. Veselova, N. N. Tarasova
Rok vydání:	2009
Předmět:	chemistry.chemical_classification biology Strain (chemistry) Sclerotinia sclerotiorum biology.organism_classification Pseudomonas chlororaphis Microbiology Rhizoctonia solani Plasmid chemistry Gene expression Genetics Lactonase biology.protein Lactone
Zdroj:	Russian Journal of Genetics. 45:30-34
ISSN:	1608-3369 1022-7954
DOI:	10.1134/s1022795409010049
Popis:	The introduction into strain Pseudomonas chlororaphis 449 of plasmid pME6863 that contains the cloned gene for N-acyl-homoserine lactonase, AiiA, leads to the degradation of all three types of N-acylhomoserine lactones produced by this strain (N-butanoyl-homoserine lactone, N-hexanoyl-homoserine lactone, and N-3-oxo-hexanoyl-homoserine lactone). This causes a drastic reduction in the synthesis of phenazine pigment and decreases the ability of cells to migrate on the surface of nutrient medium. However, the antagonistic activity of P. chlororaphis 449 toward phytopathogenic fungi Sclerotinia sclerotiorum and Rhizoctonia solani is not only decreased, but is even slightly increased; no essential changes in the exoprotease activity were observed. It is assumed that one of the QS systems of P. chlororaphis 449 may exert the repression effect on the expression of genes, which determine the two latter cell activities.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::83a1b91ef0632c0235c13d95d0374607 https://doi.org/10.1134/s1022795409010049 Zobrazit plný text záznamu Full text from SpringerLink