| Autor: |
David Barnett, llesh V Jani, Fred Mhalu, Debbie K. Glencross, George Janossy, Arabjan Iqbal, Gunnel Biberfeld, Vivian Granger, Lesley Scott, John T. Reilly, Eligius Lyamuya |
| Rok vydání: |
2001 |
| Předmět: |
|
| Zdroj: |
Journal of Immunological Methods. 257:145-154 |
| ISSN: |
0022-1759 |
| DOI: |
10.1016/s0022-1759(01)00458-6 |
| Popis: |
We tested the feasibility and precision of affordable CD4+ T cell counting in resource-poor settings using a recently standardised fixative, TransFix in whole blood (WB) by flow cytometry (FCM). The precision of the assays was established under optimal conditions for single-platform FCM such as the volumetric CytoronAbsolute and the bead-based FACSCan. Fresh WB samples from HIV-seropositive and seronegative patients were tested in Tanzania and South Africa, fixed and sent to the UK for reanalysis 7 days later. Correlation, bias and limits of agreements were analysed by linear regression and the Bland-Altman test. Absolute CD4+ T cell counts remained stable for at least 10 days when TransFix was added to WB in 1:10 dilution at 20-25 degrees C, and for 7 days when added in 1:10 or 1:5 dilution to samples stored to mimic 'tropical' conditions at 37 degrees C. Higher temperatures such as 42 degrees C were tolerated for only short periods since the recovery had decreased to 63% by day 3. The reproducibility of lymphocyte subset analysis remained unchanged by TransFix with coefficient of variations |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect