Chemical constituents and cytotoxic activity of stem bark extract of Calophyllum inophyllum
| Autor: | R.F. Kolawole, Olaoye Solomon Balogun, O.S. Ajayi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Stem bark
Chromatography biology 010405 organic chemistry Chemistry Silica gel Brine shrimp biology.organism_classification 01 natural sciences 0104 chemical sciences Calophyllum inophyllum 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Column chromatography Chemical constituents Methanol Confirmed present |
| Zdroj: | Ife Journal of Science. 21:411 |
| ISSN: | 0794-4896 |
| DOI: | 10.4314/ijs.v21i2.14 |
| Popis: | Stem bark of Calophyllum inophyllum was extracted with aqueous methanol and screened for secondary metabolites. The crude extract was partitioned with n-hexane to remove fat-soluble constituents, thereafter a portion of the defatted extract was purified using silica gel column chromatography. Both the crude and defatted extracts were investigated for cyctotoxic activity using Brine shrimp lethality assay. A number of bioactive secondary metabolites were confirmed present in the crude while the chromatographic purification afforded three compounds that were characterized as stigmasta-5, 22-dien-3-O-β-D-glucoside (C-1), macluraxanthone (C-2A) and 1, 5-dihydroxyxanthone (C-2B). LC for both crude and defatted extract were 56.22 and 183.55 50 µg/mL, respectively, indicating a good cytotoxic potential. Key words : Calophylum inophyllum ; xanthones; secondary metabolites; Brine shrimps |
| Databáze: | OpenAIRE |
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