Effects of Exogenous Bone Morphogenetic Protein 2 on the Formation of Mouse Molar Tooth Germ
| Autor: | Satonari Akutsu, Thomas G.H. Diekwisch, Tadayoshi Fukui, Ayao Hirashita, Akira Yamane, Shinichiro Oida |
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| Rok vydání: | 2004 |
| Předmět: |
Molar
H&E stain Medicine (miscellaneous) Embryo Anatomy Biology Organ culture Bone morphogenetic protein 2 Molecular biology General Biochemistry Genetics and Molecular Biology Housekeeping gene Proliferating cell nuclear antigen stomatognathic system biology.protein Immunohistochemistry General Dentistry |
| Zdroj: | Journal of Oral Biosciences. 46:530-535 |
| ISSN: | 1349-0079 |
| DOI: | 10.1016/s1349-0079(04)80028-8 |
| Popis: | The effects of exogenous bone morphogenetic protein 2 (BMP2) in the formation of mouse molar tooth germ were investigated using an organ culture system of mandibles. Mandibles dissected from mouse embryos at Ell were cultured in BGJb medium supplemented with and without 4 μg/ml of BMP2 for 8 days. The mRNA expression of glyceraldehyde-phosphate dehydrogenase (GAPDH), a housekeeping gene, in the mandibles was examined by competitive polymerase chain reaction in com bination with reverse-transcription. Cryosections of the mandibles were prepared and stained with hematoxylin and eosin, and the maximum area of molar tooth germ on the section was measured. The distribution of proliferating cell nuclear antigen (PCNA) was analyzed using immunohistochemistry. Exogenous BMP2 did not induce a significant difference in the amount of mRNA for the GAPDH contained in the mandibles. The maximum area of BMP2 - treated molar tooth germ was 0.0348 ° 0.0027 mm 2 (mean ° SD, n=4), which was 78% greater than that of the control molar tooth germ (0.0195 ° 0.0046 mm 2 ) (p |
| Databáze: | OpenAIRE |
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