Protective and therapeutic effects of Phoenix dactylifera leaf extract on pancreatic β-cells and diabetic parameters in streptozotocin-induced diabetic rats

Autor: Ebrahim Esfandiari, Aliasghar Dorali, Bahman Rashidi, Fatemeh Sadat Mostafavi, Mostafa Ghanadian
Rok vydání: 2020
Předmět:
Zdroj: Comparative Clinical Pathology. 29:847-854
ISSN: 1618-565X
1618-5641
Popis: The present study is designed to assess the impact of Phoenix dactylifera (P.d) leaf extract on plasma glucose concentration, diabetic parameters, and the number of β-cells in streptozotocin (STZ)-induced diabetic rats. In this study, diabetes was induced in male Wistar rats via intraperitoneal administration of STZ (55 mg/kg). In this study, 49 rats were divided into seven groups randomly (n = 7 per group). Our three treatment groups for 42 days were given 250 mg/kg of P.d extract, 500 mg/kg of P.d extract, and 5 mg/kg glibenclamide orally, respectively. Then, the animals were anesthetized and blood samples were obtained from puncturing the retro-orbital sinus at the initial and end of the experiment and finally sacrificed. Fasting blood glucose (FBG) levels and serum biochemical parameters including malondialdehyde (MDA), glycosylated hemoglobin (HbA1c) and insulin level were measured. Pancreatic tissue was identified by immunohistochemistry and morphometric analysis which was used to evaluate β-cells. Statistical analysis was done by applying SPSS statistical software; ANOVA and Tukey’s test were used to assess data in the control and treatment groups. Blood glucose significantly decreased after daily oral administration of P.d in diabetic rats (P
Databáze: OpenAIRE
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