Effects of clarified neem oil on growth and aflatoxin B1 formation in submerged and plate cultures of aflatoxigenicAspergillus spp

Autor: Deepak Bhatnagar, Betty Y. Shih, Hampden J. Zeringue
Rok vydání: 2001
Předmět:
Zdroj: Phytoparasitica. 29:361-366
ISSN: 1876-7184
0334-2123
DOI: 10.1007/bf02981850
Popis: An increase of 11–31% of dry mycelial mass was observed along with a slight decrease (5–10%) in aflatoxin Bi production in 5-day-old aflatoxigenicAspergillus spp. submerged cultures containing either 0.5 ml or 1.0 ml clarified neem oil (CNO) in 0.1 % Triton solution. Fungal growth and aflatoxin B1 production were also determined in potato-dextrose-agar petri plate cultures inoculated with aflatoxigenicAspergillus spp. containing an atmosphere of volatiles emitted from 0.25 ml, 0.5 ml, and 1.0 ml CNO added to the plates. After 5 days’ incubation, fungal radial growth was reduced by 7–29% and aflatoxin B1 production by 0–67%. GC/MS analysis of the head space volatiles of the CNO indicated that the reduction of fungal growth and aflatoxin B1 was probably due to low molecular weight hydrocarbons, aldehydes, alcohols, and sulfur compounds emitted at 30°C in the dry culture. These results suggest that volatiles emitted from CNO at 30° C in plate cultures were more fungistatic and consequently inhibited aflatoxin production more than neem oil added in liquid cultures.
Databáze: OpenAIRE
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