Splenic artery ligation: A protection against hepatic ischemia/reperfusion injury in partially hepatectomized rats
| Autor: | Saburo Horikawa, Koji Ito, Yumi Noda, Satoru Ikeda, Takumi Irie, Shinji Tanaka, Shigeki Arii, Hisashi Ozasa |
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| Rok vydání: | 2012 |
| Předmět: |
Liver injury
medicine.medical_specialty Hepatology business.industry Ischemia Hepatoduodenal ligament Spleen Splenic artery medicine.disease Gastroenterology Surgery Heme oxygenase Infectious Diseases medicine.anatomical_structure Internal medicine medicine.artery medicine business Ligation Reperfusion injury |
| Zdroj: | Hepatology Research. 42:819-827 |
| ISSN: | 1386-6346 |
| Popis: | Aim In liver resection, the temporary occlusion of the hepatoduodenal ligament (Pringle maneuver) is often used. However, the maneuver causes ischemia/reperfusion (I/R) injury in the remnant liver. Heme oxygenase (HO)-1 has a cytoprotective role against this injury. Our aim is to investigate whether splenic artery ligation induces HO-1 expression in the liver and ameliorates the hepatic I/R injury in partially hepatectomized rats. Methods Rats underwent splenic artery ligation by occluding the main splenic artery. Two days later, the total hepatic ischemia (Pringle maneuver) was conducted, and then a two-thirds partial hepatectomy (PH) was performed just before the start of reperfusion. HO inhibitor was twice injected s.c. at 3 and 16 h before the Pringle maneuver. HO-1 levels were determined by western blotting. Liver injury was biochemically assessed. Results In normal rats, HO-1 was highly expressed in the spleen, but not in the liver. Splenic artery ligation induced HO-1 in the livers. When rats underwent 20 and 30 min of Pringle maneuver/PH, survival rates were 28% and 8%, respectively. Splenic artery ligation significantly improved both the survival rates: 73% and 56%, respectively. Under these conditions, administration of HO-1 inhibitor at least partly negated the efficacy of splenic artery ligation. Splenic artery ligation also increased the recovery rate of the remnant liver mass and platelet counts in Pringle maneuver/PH-treated rats. Conclusion Splenic artery ligation was significantly effective on the hepatic I/R injury in partially hepatectomized rats. Induction of HO-1 may be at least partly involved in the improvement of this injury. |
| Databáze: | OpenAIRE |
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