Mining the effector repertoire of the biotrophic fungal pathogen Ustilago hordei during host and non-host infection
| Autor: | Daniel Mathow, Bilal Ökmen, Daniela Aßmann, Urs Lahrmann, Gunther Doehlemann, Alexander Hof |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetics medicine.medical_specialty Ustilago hordei Effector food and beverages Soil Science Virulence Genomics Plant Science Biology biology.organism_classification 01 natural sciences Transcriptome 03 medical and health sciences Pathosystem 030104 developmental biology Molecular genetics medicine Agronomy and Crop Science Molecular Biology Gene 010606 plant biology & botany |
| Zdroj: | Molecular Plant Pathology. 19:2603-2622 |
| ISSN: | 1464-6722 |
| Popis: | The success of plant-pathogenic fungi mostly relies on their arsenal of virulence factors which are expressed and delivered into the host tissue during colonization. The biotrophic fungal pathogen Ustilago hordei causes covered smut disease on both barley and oat. In this study, we combined cytological, genomics and molecular biological methods to achieve a better understanding of the molecular interactions in the U. hordei-barley pathosystem. Microscopic analysis revealed that U. hordei densely colonizes barley leaves on penetration, in particular the vascular system. Transcriptome analysis of U. hordei at different stages of host infection revealed differential expression of the transcript levels of 273 effector gene candidates. Furthermore, U. hordei transcriptionally activates core effector genes which may suppress even non-host early defence responses. Based on expression profiles and novelty of sequences, knockout studies of 14 effector candidates were performed in U. hordei, which resulted in the identification of four virulence factors required for host colonization. Yeast two-hybrid screening identified potential barley targets for two of the effectors. Overall, this study provides a first systematic analysis of the effector repertoire of U. hordei and identifies four effectors (Uvi1-Uvi4) as virulence factors for the infection of barley. |
| Databáze: | OpenAIRE |
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