Cloning, quantification and characterization of a minisatellite DNA sequence from common bean Phaseolus vulgaris L

Autor:	R. Jalouzot, B. Hamon, Didier Peltier, I. Métais, Catherine Aubry
Rok vydání:	1998
Předmět:	Genetics Nucleic acid sequence food and beverages General Medicine Biology biology.organism_classification Molecular biology genomic DNA chemistry.chemical_compound Minisatellite DNA profiling chemistry Genetic marker Phaseolus Agronomy and Crop Science DNA Biotechnology Genomic organization
Zdroj:	Theoretical and Applied Genetics. 97:232-237
ISSN:	1432-2242 0040-5752
Popis:	We describe the cloning and the characterization of a 130-bp DNA fragment, called OPG9-130, amplified from bean (Phaseolus vulgaris L.) genomic DNA. This fragment corresponds to a minisatellite DNA sequence containing seven repeats of 15 bp which differ slightly from each other in their sequence. Southern analysis showed that the core sequence of 15 bp is repeated in clusters dispersed throughout the genome. The use of this fragment as a probe allowed us to identify common bean lines by their DNA fingerprints. We suggest that OPG9-130 will be useful for line identification as well as for the analysis of genetic relatedness between bean species and lines.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::834eca00fa9437f8d6a8a5e6483e8713 https://doi.org/10.1007/s001220050890 Zobrazit plný text záznamu Full text from SpringerLink