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Fluorescence spectroscopy is an inherently selective technique; however, single wavelength measurements can be limited for the analysis of complicated multicamponent samples or even a simple mixture with overlapping emission spectra. To overcome this limitation, multidimensional fluorescence measurements can beused to obtain additional information about the sample. The multidimensionalfluorescence measurement involves the use of multiple parameters to increase thespecificity of the measurement. In this laboratory, we are especially interestedin multiparametric measurements such as the Excitation -Emission Spectrum.In this paper a fiber optic based multidimensional fluorometer is examined along with a portable multichannel fluorometer in terms of sensitivity. Inaddition, specific applications incorporating the excitation- emission matrix arepresented. i. INTRODUCTION 1.1 MULTIDIMENSIONAL FLUOR The acceptance of fluorescence spectroscopy as an analytical technique is well documented in several texts 1 -3. |
