| Popis: |
Last-resort antibiotics act as ultimate force to overcome multidrug-resistant strains infections. Cases of tigecycline resistance in gram-negative bacilli in clinical settings are reported worldwide, however, there is no data related to tigecycline resistant strains in river water. This study demonstrates seven tigecycline gram-negative bacilli isolated from river water in Rio de Janeiro metropolitan area, their resistance genes, ability of biofilm formation with/without antibiotics and behavior using the nematode Caenohabidits elegans as infection in vivo model. From 24 gram-negative isolated strains, 16 (66.6%) were classified as multidrug-resistant, however, seven (29.1%) presented resistant to all antimicrobial agents tested, including tigecycline and have been identified by MALDI-TOF as A. baumannii, E. aerogenes and P. agglomerans. All tigecycline-resistant strains presented amplification products for ESBL, AME and PMQR and ability of biofilm formation on hydrophilic and hydrophobic abiotic surfaces with and without antimicrobial agents. The presence of antimicrobials did not inhibit biofilm formation. Tigecycline-resistant strains differed of OP50 control with P |
