Influence of spacers and organic modifiers on chromatographic behaviors on chiral diamide stationary phase with N-(3,5-dimethylbenzoyl)-d-phenylglycine

Autor:	Zilin Chen, Toshiyuki Hobo, Takashi Fuyumuro, Katsunori Watabe
Rok vydání:	2004
Předmět:	chemistry.chemical_classification Chromatography Silica gel Chemical modification Biochemistry Analytical Chemistry Amino acid chemistry.chemical_compound Silanol chemistry Stationary phase Environmental Chemistry Organic chemistry Amine gas treating Spectroscopy D-phenylglycine Isopropyl
Zdroj:	Analytica Chimica Acta. 518:181-189
ISSN:	0003-2670
DOI:	10.1016/j.aca.2004.05.039
Popis:	A chiral diamide stationary phase (CSP) with different length of spacers has been synthesized by chemically bonding the N -(3,5-dimethylbenzoyl)- d -phenylglycine on the aminopropylsilica gel or silica gel bonded with different carbon chain length of spacers. These CSPs have successfully been used for the chiral resolutions of the benzoyl- dl -octylamine and benzoyl- dl -amino acid isopropyl esters in normal-phase liquid chromatography (NP-LC). The spacer length and the introduction of a secondary amine group in the CSPs have been investigated. It has been found that a long spacer of CSPs generally benefits on the enantioselectivity. The introduction of secondary amine group in the spacer leads to the decline of separation factors. Further, the effect of organic modifiers has been examined. Proper use of organic modifiers resulted in an interesting ‘masking effect’ on the residing silanol groups of the CSPs. The addition of suitable amount of organic modifiers improves the enantioselectivity, and enantioselectivity depends on the shape of organic modifiers in the mobile phases.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::831c68510eb4333fd95eb89e480a4ba9 https://doi.org/10.1016/j.aca.2004.05.039 Zobrazit plný text záznamu Full Text from ScienceDirect