Affinity Mesh Screen Materials for Selective Extraction and Analysis of Antibiotics Using Transmission Mode Desorption Electrospray Ionization Mass Spectrometry
| Autor: | Sumit Bhawal, Rajendrasing Deshmukh, Kevin A. Schug, John A. Gurak, Richard B. Timmons, Brian L. Edwards, Aruna B. Wijeratne, Frank W. Foss, Samuel H. Yang, Evelyn H. Wang |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Desorption electrospray ionization Chromatography Carboxylic acid Peptide Surfaces and Interfaces Polymer Condensed Matter Physics Condensation reaction Mass spectrometry Combinatorial chemistry Plasma polymerization chemistry.chemical_compound chemistry Electrochemistry General Materials Science Spectroscopy Acrylic acid |
| Zdroj: | Langmuir. 29:8046-8053 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | The extraction of active compounds from natural sources has shown to be an effective approach to drug discovery. However, the isolation and identification of natural products from complex extracts can be an arduous task. A novel approach to drug discovery is presented through the use of polymer screens functionalized with an l-lysine-d-alanine-d-alanine (Kaa) peptide to create new affinity capture mesh screen materials. The Kaa sequence is a well-characterized specific binding site for antibiotics that inhibit cell wall synthesis in Gram-positive bacteria. The detailed synthesis and characterization of these novel screen materials are presented in this work. Polypropylene mesh screens were first coated with a poly(acrylic acid) film by pulsed plasma polymerization. The synthesized Kaa peptide was then covalently attached to carboxylic acid groups through a condensation reaction. An analysis of captured compounds was performed in a rapid fashion with transmission-mode desorption electrospray ionization (TM... |
| Databáze: | OpenAIRE |
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