Popis: |
Capillary isotachophoresis has been developed and modified for use as a preparative method. The sample zones are pushed out of the capillary under voltage by a counter-flow of leading electrolyte through a small T-tube connection immediately after the UV detector. The zones are collected on a moving cellulose acetate strip without loss of high resolution. This permits simple detection and identification by methods new to capillary isotachophoresis, e.g. , immunological and radioactive methods. The zymogram technique can be used directly on the strip. The preparative procedure also makes capillary isotachophoresis a powerful purification method. |