Preparation of Close-Packed Silver Nanoparticles on Graphene to Improve the Enzyme Immobilization and Electron Transfer at Electrode in Glucose/O2 Biofuel Cell
| Autor: | Fengjin Qu, Xiaoyan Ma, Yan Gao, Yuchen Hui, Fang Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Immobilized enzyme
biology Chemistry Nanotechnology 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences Silver nanoparticle 0104 chemical sciences Anode Electron transfer Chemical engineering Electrode biology.protein Glucose oxidase 0210 nano-technology Enzymatic biofuel cell |
| Zdroj: | Chinese Journal of Chemistry. 35:1098-1108 |
| ISSN: | 1001-604X |
| Popis: | In this study, chemical reduced graphene-silver nanoparticles hybrid (AgNPs@CR-GO) with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between immobilized enzyme and electrode. A facile route to prepare AgNPs@CR-GO was designed involving in β-cyclodextrin (β-CD) as reducing and stabilizing agent. The morphologies of AgNPs were regulated and controlled by various experimental factors. To fabricate the bioelectrode, AgNPs@CR-GO was modified on glassy carbon electrode followed by immobilization of glucose oxidase (GOx) or laccase. It was demonstrated by electrochemical testing that the electrode with close-packed AgNPs provided high GOx loading (Γ=4.80 × 10−10 mol•cm−2) and fast electron transfer rate (ks=5.76 s−1). By employing GOx based-electrode as anode and laccase based-electrode as cathode, the assembled enzymatic biofuel cell exhibited a maximum power density of 77.437 μW•cm−2 and an open-circuit voltage of 0.705 V. |
| Databáze: | OpenAIRE |
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