Use of the 2-chlorotrityl chloride resin for microwave-assisted solid phase peptide synthesis
| Autor: | Kirsty Elizabeth High, Theodore Tselios, Matthaia Ieronymaki, Kirsty Penkman, Dimitrios Gatos, Anna Pantelia, Irene Friligou, Maria Eleni Androutsou, Molly Crisp |
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| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Organic Chemistry Biophysics General Medicine Biochemistry Chloride Combinatorial chemistry Cyclic peptide Amino acid Biomaterials chemistry.chemical_compound chemistry Phase (matter) Peptide synthesis medicine Solid-Phase Synthesis Techniques Organic chemistry Racemization Microwave medicine.drug |
| Zdroj: | Biopolymers. 104:506-514 |
| ISSN: | 0006-3525 |
| Popis: | A fast and efficient microwave (MW)-assisted solid-phase peptide synthesis protocol using the 2-chlorotrityl chloride resin and the Fmoc/tBu methodology, has been developed. The established protocol combines the advantages of MW irradiation and the acid labile 2-chlorotrityl chloride resin. The effect of temperature during the MW irradiation, the degree of resin substitution during the coupling of the first amino acids and the rate of racemization for each amino acid were evaluated. The suggested solid phase methodology is applicable for orthogonal peptide synthesis and for the synthesis of cyclic peptides. |
| Databáze: | OpenAIRE |
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