Establishment of Agrobacterium rhizogenes-mediated hairy root transformation of Crocus sativus L
| Autor: | Shilpi Sharma, Jyoti Vakhlu, Praveen Kumar Verma, Yeshveer Singh |
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| Rok vydání: | 2021 |
| Předmět: |
Reporter gene
biology Agrobacterium ved/biology fungi ved/biology.organism_classification_rank.species food and beverages Environmental Science (miscellaneous) biology.organism_classification Agricultural and Biological Sciences (miscellaneous) Molecular biology Transformation (genetics) Plasmid Crocus sativus Gene Biotechnology Transformation efficiency Crocus |
| Zdroj: | 3 Biotech. 11 |
| ISSN: | 2190-5738 2190-572X |
| DOI: | 10.1007/s13205-020-02626-2 |
| Popis: | Efficient transformation system for genetic improvement is essential in Crocus sativus, as it lacks sexual reproduction. This is the first report wherein an efficient protocol is developed for the transformation of Crocus sativus L. by Agrobacterium rhizogenes strain ARqua1 with a transformation efficiency of 78.51%. The ARqua1 strain harboring both Ri plasmid and binary vector plasmid pSITE-4NB, and marker genes for red fluorescent protein (RFP) and a β-glucuronidase (GUS) reporter gene were used for selection. Transformation was confirmed by RFP signal, GUS reporter assay and polymerase chain reaction (PCR) analysis of the test samples after 21 days post inoculation. These results confirm the establishment of protocol for hairy root transformation in C. sativus that can be further used for gene transfer or gene editing in Crocus for its genetic improvement. |
| Databáze: | OpenAIRE |
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