Microstructure of bacterial poly(?-hydroxybutyrate-co-?-hydroxyvalerate) by FAB mass-spectrometry
| Autor: | R. H. Marchessault, F. G. Morin, Maria Elena Nedea |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
chemistry.chemical_classification
Chromatography Polymers and Plastics General Chemistry Polymer Nuclear magnetic resonance spectroscopy Fast atom bombardment Condensed Matter Physics Mass spectrometry High-performance liquid chromatography Oligomer chemistry.chemical_compound Monomer chemistry Materials Chemistry Chemical composition |
| Zdroj: | Polymer Bulletin. 26:549-556 |
| ISSN: | 1436-2449 0170-0839 |
| Popis: | Bacterially synthesized Poly (β-hydroxybutyrate-co-β-hydroxyvalerate), P(HB-co-HV), with different compositions were analyzed by NMR spectroscopy and HPLC-mass spectrometry. Partial methanolysis or partial ammonolysis of P(HB-co-HV) has been performed, the oligomers obtained were separated by high-performance liquid chromatography (HPLC) and identified by fast atom bombardment mass spectrometry, (FAB-MS). The normalized intensities of oligomer peaks provided an estimate of the copolymer composition and of the sequence distribution of monomeric units. The interpretation of the data indicated that the sequence distributions of all samples were statistically random (Bernoullian model), and permited the detection in one sample, of traces of pure poly(β-hydroxybutyrate), (PHB). |
| Databáze: | OpenAIRE |
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