Acetylcholine modulates K + and Na + currents in human basal forebrain cholinergic neuroblasts through an autocrine/paracrine mechanism

Autor: Renato Corradetti, Erica Sarchielli, Pasquale Gallina, Annamaria Morelli, Elisabetta Coppi, Gabriella B. Vannelli, Irene Fusco, Anna Maria Pugliese, Giulia Guarnieri, Federica Cherchi, Felicita Pedata
Rok vydání: 2020
Předmět:
Zdroj: Journal of Neurochemistry. 157:1182-1195
ISSN: 1471-4159
0022-3042
Popis: The Nucleus Basalis of Meynert (NBM) is the main source of cholinergic neurons in the basal forebrain to be crucially involved in cognitive functions and whose degeneration correlates with cognitive decline in major degenerative pathologies as Alzheimer's and Parkinson's diseases. However, knowledge concerning NBM neurons derived from human brain is very limited to date. We recently characterized a primary culture of proliferating neuroblasts isolated from the human fetal NBM (hfNBM) as immature cholinergic neurons expressing the machinery to synthetize and release acetylcholine. Here we studied in detail electrophysiological features and cholinergic effects in this cell culture by patch-clamp recordings. Our data demonstrate that atropine-blocked muscarinic receptor activation by acetylcholine or carbachol enhanced IK and reduced INa currents by stimulating Gi -coupled M2 or phospholipase C-coupled M3 receptors, respectively. Inhibition of acetylcholine esterase activity by neostigmine unveiled a spontaneous acetylcholine release from hfNBM neuroblasts that might account for an autocrine/paracrine signaling during human brain development. Present data provide the first description of cholinergic effects in human NBM neurons and point to a role of acetylcholine as an autocrine/paracrine modulator of voltage-dependent channels. Our research could be of relevance in understanding the mechanisms of cholinergic system development and functions in the human brain, either in health or disease.
Databáze: OpenAIRE
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