Column chromatography and immunoassay compared for measuring the isoenzymes of aspartate aminotransferase in serum

Autor:	W H Hannon, S A Miller, S S McKneally, V S Whitner, C McKenzie, C A Burtis, Eric J. Sampson
Rok vydání:	1979
Předmět:	Chromatography medicine.diagnostic_test Biochemistry (medical) Clinical Biochemistry Aspartate Aminotransferases Biology Isozyme Molecular biology Standard error Column chromatography Immunoassay method Homogeneous Immunoassay medicine
Zdroj:	Clinical Chemistry. 25:1691-1695
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/25.10.1691
Popis:	We compare a column-chromatographic method and a homogeneous immunoassay method for separately measuring the mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase. Analytical recovery for the two methods averaged 102% (SD, 2%) and 103% (SD, 4%), respectively, for 11 pools prepared by adding the purified isoenzymes to serum and 102% (SD 8.9%) and 89% (SD, 8.1%) for 26 unaltered specimens of human serum. In comparing the results of the immunoassay method (y) to the chromatographic method (x), our measurements agreed closely for the mitochondrial (y = 0.947 X + 7, r = 0.9991, standard error of estimate = 2.9 U/L) and cytoplasmic (y = 0.92x-6, r = 0.9995, standard error of estimate = 2.1 U/L) isoenzymes in pools prepared from the purified isoenzymes. Similar measurements of the 26 human serum specimens yielded the following results for least-squares evaluation; cytoplasmic isoenzyme y = 1.03x-11, r = 0.994, and standard error of estimate = 6.0 U/L; mitochondrial isoenzyme y = 0.75x+0, r = 0.927, and standard error of estimate = 3.9 U/L.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::82f0f189e627ec5eebb73dd953db1ce6 https://doi.org/10.1093/clinchem/25.10.1691 Zobrazit plný text záznamu