Enhanced DNA Cleavage by Mercury(II) Porphyrin at a Low Concentration of HaeIII Restriction Enzyme

Autor:	Elvis Nyarko, Keiichi Watanabe, Masaaki Tabata
Rok vydání:	2001
Předmět:	chemistry.chemical_element Free base General Chemistry Porphyrin Mercury (element) HaeIII chemistry.chemical_compound Restriction enzyme chemistry Dna cleavage Cleave Polymer chemistry polycyclic compounds medicine Organic chemistry DNA medicine.drug
Zdroj:	Chemistry Letters. 30:932-933
ISSN:	1348-0715 0366-7022
DOI:	10.1246/cl.2001.932
Popis:	Mercury(II) porphryin enhanced DNA cleavage in the presence of 0.2 units µL−1 of HaeIII at which concentration the restriction enzyme could not cleave DNA in the absence of the porphyrin. This was ascribed to the synergistic effect of the bound Hg2+ ions to DNA and the intercalated free base porphyrin, released from the mercury(II) porphyrin complex upon binding to DNA, which was confirmed by UV–visible and CD spectroscopic measurements.
Databáze:	OpenAIRE
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