Chimeric Aptamers-Based and MoS2 Nanosheet-Enhanced Label-Free Fluorescence Polarization Strategy for Adenosine Triphosphate Detection
Autor: | Jing Zhang, Zhao-Li Mou, Fuquan Dang, Yao-Yao Fan, Jun Li, Zhi-Qi Zhang, Man Wang |
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Rok vydání: | 2018 |
Předmět: |
Chemistry
Aptamer 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Fluorescence 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Berberine Biophysics 0210 nano-technology Bifunctional Enhancer Adenosine triphosphate DNA Fluorescence anisotropy |
Zdroj: | Analytical Chemistry. 90:13708-13713 |
ISSN: | 1520-6882 0003-2700 |
Popis: | Adenosine triphosphate (ATP) as a primary energy source plays a unique role in the regulation of all cellular events. The necessity to detect ATP requires sensitive and accurate quantitative analytical strategies. Herein, we present our study of developing a MoS2 nanosheet-enhanced aptasensor for fluorescence polarization-based ATP detection. A bifunctional DNA strand was designed to consist of chimeric aptamers that recognize and capture ATP and berberine, a fluorescence enhancer. In the absence of ATP, the DNA strand bound to berberine will be hydrolyzed when Exonuclease I (Exo I) is introduced, releasing berberine as a result. In contrast, when ATP is present, ATP aptamer folds into a G-quadruplex structure; thus, the complex can resist degradation by Exo I to maintain berberine for fluorescent detection purpose. In addition, to magnify the fluorescence polarization (FP) signal, MoS2 nanosheets were also adopted in the system. This nanosheets-enhanced FP strategy is simple and facile which does not req... |
Databáze: | OpenAIRE |
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