Genetic Polymorphisms HLA Class II in SIRS and Sepsis in Children
| Autor: | E. Hagina, Dace Gardovska, Arturs Sochnevs, E. Eglite, Jana Pavare, Linda Eihvalde, Ilze Grope |
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| Rok vydání: | 2014 |
| Předmět: |
musculoskeletal diseases
Genetics medicine.medical_specialty Environmental Engineering business.industry Haplotype Single-nucleotide polymorphism Odds ratio medicine.disease Gastroenterology Industrial and Manufacturing Engineering Sepsis symbols.namesake Statistical significance Internal medicine Genotype medicine symbols skin and connective tissue diseases business HLA-DRB1 Fisher's exact test |
| Zdroj: | British Journal of Medicine and Medical Research. 4:149-160 |
| ISSN: | 2231-0614 |
| Popis: | Aims: The aim of this research was to investigate the genetically determined predisposition to develop SIRS and sepsis by analyzing human leukocyte antigen HLA class II genes. Study Design: children defined by the criteria for SIRS of Critical Care Medicine Consensus Conference were included into the study in a prospective manner. Place and Duration of Study: Riga Stradiņs University Laboratory of Clinical Immunology and Immunogenetic, Department of Pediatrics, Rīga Stradiņs University, and Children’s Clinical University Hospital, Latvia, between January 2008 and May 2009. Methodology: Samples from children with SIRS and sepsis were collected at the Children`s Clinical University Hospital of Latvia. During the study, 65 patients with SIRS were observed. In 12 cases among SIRS patients, sepsis was confirmed. DNA was separated from lymphocytes of peripheral blood. At the same time, 100 DNA samples Research Article British Journal of Medicine & Medical Research, 4(1): 149-160, 2014 150 from healthy children were analysed. HLA typing low-resolution for HLADRB1; DQB1; DQA1 was performed by polymerase chain reaction (PCR) with amplification with sequence-specific primers (SSP). PCR products were separated on 3% arose, the amplified bands were visualised. The frequency of alleles and genotypes were compared between the patients and the controls using chi-square test. P-value and odds ratio were calculated using EPI INFO software version 6 with 95 % confidence intervals and Fisher exact correction for small numbers. Results: The frequency of DRB1*07:01 allele was significantly increased in patients with SIRS (OR=8.71; 95% Cl = 2.8-26.8; p |
| Databáze: | OpenAIRE |
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