Low‐shear Sodding of an Implantable Expanded Polytetrafluoroethylene Scaffold with a Rat Adipose‐derived Stromal‐vascular Fraction

Autor:	Philip Gastrell, Bruce Klitzman, Jinny Cho
Rok vydání:	2015
Předmět:	education.field_of_study Cell growth Chemistry Cell Population Adipose tissue Stromal vascular fraction Biochemistry Cell biology medicine.anatomical_structure Genetics Collagenase medicine Stem cell Wound healing education Molecular Biology Biotechnology medicine.drug
Zdroj:	The FASEB Journal. 29
ISSN:	1530-6860 0892-6638
DOI:	10.1096/fasebj.29.1_supplement.lb650
Popis:	Impaired wound healing occurs in various pathologies and is characterized by diminished substrate delivery, inadequate cellular proliferation and tissue fibrosis. The adipose-derived stromal-vascular fraction (SVF) cell population – primarily adipose-derived stem cells, fibroblasts and endothelial cells – is uniquely suited to rescue impaired wound healing and tissue regeneration. The SVF cells have been shown to inhibit tissue fibrosis, improve local cell proliferation and induce neomicrovascularization, which improves substrate delivery. One of the challenges of using adipose-derived SVF cells to improve wound healing is finding a reliable, reproducible method of delivering this cell population into the target tissue bed. A possible approach entails attaching the cell population to an implantable porous scaffold. In this study, we isolated the SVF by harvesting the epididymal fat pad of male Sprague Dawley rats. This fat was then minced, digested with .075% collagenase and centrifuged to isolate a cellu...
Databáze:	OpenAIRE
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