| Autor: |
Jeffrey W. Roberts, Jeffrey A. Goliger |
| Rok vydání: |
1987 |
| Předmět: |
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| Zdroj: |
Journal of Biological Chemistry. 262:11721-11725 |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(18)60870-1 |
| Popis: |
Phage 82 gene Q encodes a phage-specific positive regulator of late gene expression, thought, by analogy to the corresponding gene of phage lambda, to be a transcription antiterminator. We have cloned and sequenced the phage 82 gene Q and have overproduced and purified the 82 Q protein. We also have identified and sequenced DNA containing the phage 82 late gene promoter and terminator. We show that purified 82 Q protein is active and specific for DNA containing the 82 late gene promoter in a well defined in vitro transcription reaction: RNA polymerase initiating at the phage 82 late gene promoter and modified by 82 Q protein reads through a downstream transcriptional terminator. We used T1 RNase mapping to confirm that the putative readthrough RNA made in the presence of 82 Q protein is in fact an elongation product of the shorter RNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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