Experimental and theoretical studies of interaction of aliphatic chain α-aminobisphosphonates with DNA
| Autor: | Hossein Peyman, Kh. Gholivand, Mohammad Bagher Gholivand, Avat Arman Taherpour, Rouhollah Yaghoubi, Hamideh Roshanfekr |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Circular dichroism Molecular model General Chemical Engineering General Physics and Astronomy General Chemistry Electrochemistry Photochemistry Chloride Fluorescence 03 medical and health sciences Crystallography chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine chemistry 030220 oncology & carcinogenesis medicine Moiety Alkyl DNA medicine.drug |
| Zdroj: | Journal of Photochemistry and Photobiology A: Chemistry. 338:183-191 |
| ISSN: | 1010-6030 |
| DOI: | 10.1016/j.jphotochem.2017.01.033 |
| Popis: | The interaction of DNA with four aliphatic chain α-aminobisphosphonate were investigated in a tris–hydrogen chloride buffer (pH 7.4) in the presence and absence of spectroscopic probes (Neutral Red dye (NR) and Hoechest (Ho)) by fluorescence, UV–Vis absorption and circular dichroism (CD) spectroscopic methods, electrochemical techniques and viscometry. The spectroscopic and voltammetric studies showed that the groove binding mode of interaction is predominant in the solution containing DNA and α-aminobisphosphonates. Furthermore, the results indicated that the binding strength of the DNA–α-aminobisphosphonate complexes are dependent on alkyl moiety presented in the structure of α-aminoalkylphosphonic acids A1–A4. The principal component analysis (PCA) and theoretical quantum mechanical and molecular mechanics (QM-DFT/B3LYP/6-31+G* and MM-SYBYL) methods were also applied to determine the number of chemical components presented in complexation equilibrium and identify the structure complexes of DNA with α-aminobisphosphonates, respectively. |
| Databáze: | OpenAIRE |
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