Enantioseparation and quantitative determination of ibuprofen using vancomycin-mediated capillary electrophoresis combined with molybdenum disulfide-assisted dispersive solid-phase extraction: optimization using experimental design

Autor: Elahe Naghdi, Jahan B. Ghasemi, Ali Reza Fakhari
Rok vydání: 2020
Předmět:
Zdroj: Journal of the Iranian Chemical Society. 17:1467-1477
ISSN: 1735-2428
1735-207X
DOI: 10.1007/s13738-020-01874-6
Popis: A dispersive solid-phase extraction procedure using molybdenum disulfide as an adsorbent was developed for pretreatment and preconcentration of low quantity of ibuprofen in natural samples prior to enantioseparation. The chiral separation was carried out by vancomycin-mediated capillary electrophoresis coupled with UV detector. The effect of the main factors on the extraction recovery and enantioselective resolution was investigated using a central composite design. The best enantioseparation of ibuprofen was achieved on an uncoated capillary at 20 °C using phosphate buffer (pH 6.5) containing 1 mM vancomycin and 20 kV applied voltage. The optimum condition of the dispersive solid-phase extraction was obtained at the sample solution with pH 4.0 containing 1.4 M NaCl, 0.12 g adsorbent, 10 min adsorption time, 25 °C adsorption temperature, 1 mL the elution solvent (acetone-0.25 M NaOH (aq) (2:1 (v v−1)), 5 min desorption time, 50 °C desorption temperature, and the stirring speed of 900 rpm for adsorption and desorption steps. Good values of linearity range (0.125–5 µg mL−1, R2 = 0.999), preconcentration factor (33.4), precision (less than 2.3%), and recovery (93.2%) were achieved. The limit of detection and quantification of, respectively, 0.025 and 0.070 μg mL−1 was provided, which enabled a satisfactory trace determination of ibuprofen in urban water and human urine samples.
Databáze: OpenAIRE
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