| Autor: |
Basdeo Kushwaha, Neha Singh, Ravindra Kumar, Vijay Kumar Singh, Akhilesh Kumar Mishra, Pankaj Soni, Murali Sanjeev Kumar, Shreya Srivastava |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Turkish Journal of Fisheries and Aquatic Sciences. 21:95-105 |
| ISSN: |
1303-2712 |
| Popis: |
Schizothorax richardsonii, commonly called snow trout is a vulnerable coldwater fish distributed in India and adjoining countries, is an important resource as food, ornamental and game fishery. A cell line, designated as SREM-1, has been established from the eye muscle of S. richardsonii using explant method and cultured in Leibovitz’s L-15 medium. The growth studies at 20-32°C temperatures and 5-20% of fetal bovine serum (FBS) concentrations revealed that SREM-1 cells exhibited optimal growth at 28°C in L-15 medium containing 20% FBS. Cell type was confirmed primarily as epithelial in nature by immuno-phenotyping assay and was sub-cultured for >55 times since its development. The origin of the cell line was confirmed by sequencing of cytochrome oxidase c subunit I and 16S rRNA genes. SREM-1 cell line is free from mycoplasma contamination and has been cryopreserved at different passage levels with revival efficiency of 70-80% after 6 months. The SREM-1 cell line was successfully transfected and also employed for evaluating in vitro cytotoxicity against a heavy metal, mercuric chloride. The newly established cell line of this vulnerable species would be useful as a model for aquatic toxicological and transfection studies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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