Micro-spectrophotometry applied to intracellular pH determination

Autor:	H. Sun, J. C. Bernengo, F. Jacquey
Rok vydání:	1992
Předmět:	Absorbance Micro-spectrophotometry Chromatography Titration curve medicine.diagnostic_test Chemistry Intracellular pH Spectrophotometry Calibration Biophysics medicine Ph measurement Cellular biophysics
Zdroj:	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society.
DOI:	10.1109/iembs.1992.5760862
Popis:	This paper presents intra-cellular pH measurements, performed through absorbance technique. Calibration data on rat myoballs have been obtained with a specially designed micro-spectro-photometer. Dye-protein interactions have been investigated, and dye titration curves inside living cells are presented. Absolute cytoplasmic pH determination are shown to be possible with an accuracy of 0.02 pH unit.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::829d84aaa3b933584432d9b7476cc727 https://doi.org/10.1109/iembs.1992.5760862 Zobrazit plný text záznamu