Bacterial biofilms resist oxidising agents due to the presence of organic matter
| Autor: | G. Kunová, Vladimir Babak, H. Vlkova, Z. Jaglic, D. Cervinkova, E. Michu |
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| Rok vydání: | 2012 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chloramine 030106 microbiology Biofilm food and beverages chemistry.chemical_element biochemical phenomena metabolism and nutrition Bacterial growth Microbiology 03 medical and health sciences chemistry.chemical_compound Benzalkonium chloride chemistry Sodium hypochlorite Peracetic acid medicine Chlorine Organic matter Food Science medicine.drug |
| Zdroj: | Czech Journal of Food Sciences. 30:178-187 |
| ISSN: | 1805-9317 1212-1800 |
| Popis: | Jaglic Z., Cervinkova D., Vlkova H., Michu E., Kunova G., Babak V. (2012): Bacterial biofilms resist oxidising agents due to the presence of organic matter. Czech J. Food Sci., 30: 178–187. This study evaluated the susceptibility of planktonic and biofilm cells of Staphylococcus spp. (n = 87), klebsiella spp. (n = 30), and escherichia coli (n = 74) isolates originating from food contact surfaces of milk and meat processing plants to benzalkonium chloride (BAC), sodium hypochlorite (NaClO), chloramine B (CAB), and peracetic acid (PAA). Bacterial growth and reduction of viable cells in the presence of disinfectants were determined in tryptone soya broth (TSB) and water, respectively. Biofilm positive isolates ( n = 73) were tested for the presence of selected qac genes. Unlike BAC, chlorine‑based disinfectants and PAA were poorly efficient in TSB, especially in the case of biofilms. However, when tested in water, the efficacy of NaClO, CAB and PAA substantially increased, which was particularly evident in biofilms. In water, staphylococcal biofilms were even more susceptible to CAB than planktonic cells. A part (23.3%) of the biofilm positive staphylococci carried the qac genes but did not express an increased resistance to BAC. This study showed that bacterial biofilms protected with organic matter could be one of the main reasons for disinfection failure. |
| Databáze: | OpenAIRE |
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