| Autor: |
Alexandra C. Maschino, Lan L. Gellert, Hikmat Al-Ahmadie, Bernard H. Bochner, Philip H. Kim, Geoffrey Gotto, John P. Sfakianos |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Urologic Oncology: Seminars and Original Investigations. 32:657-662 |
| ISSN: |
1078-1439 |
| DOI: |
10.1016/j.urolonc.2014.02.003 |
| Popis: |
Objectives The PI3k/Akt pathway has been associated with the development and progression of bladder tumors, with most studies focused on papillary or muscle-invasive tumors. We sought to characterize the expression patterns of the PI3K/Akt pathway in a large cohort of high-risk preinvasive carcinoma in situ (CIS) tumors of the bladder. Our goal was to understand whether PI3K/Akt pathway alterations associated with CIS resemble early- or late-stage bladder cancers. Material and methods We evaluated tissue specimens from 97 patients with CIS of the bladder, of which 14 had a concomitant papillary tumor. All patients were treated with intravesical bacillus Calmette-Guerin. All specimens were evaluated for PTEN, p-AKT, and p-S6 immunoreactivity. Markers were evaluated for percentage and intensity of staining and were scored using a 0 to 3+grading system. Results PTEN staining was noted as least intense in 67% of tumor specimens and 22% of normal urothelium. P-Akt and p-S6 had intense staining in 77% and 90% of tumor specimens vs. 44% and 68% in normal tissue, respectively. Low-intensity staining for PTEN at 12 months correlated with higher recurrence risk ( P = 0.026). Conclusion We describe a large cohort of CIS bladder tumors with decreased staining intensity of PTEN and increased staining intensity of p-AKT and p-S6, similar to high-grade and high-stage papillary tumors. Low-intensity staining of PTEN at 12 months was associated with an increased risk of recurrence. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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