Colorimetric detection of cysteine based on dispersion–aggregation mechanism of chitosan stabilized gold nanoparticles
| Autor: | Esther Jeyasekaran, Sridevi Venkatachalam |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Organic Chemistry 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology Sulfur containing 01 natural sciences Catalysis 0104 chemical sciences Amino acid Chitosan chemistry.chemical_compound Colorimetric sensor chemistry Colloidal gold 0210 nano-technology Dispersion (chemistry) Cysteine Nuclear chemistry |
| Zdroj: | Canadian Journal of Chemistry. 97:233-237 |
| ISSN: | 1480-3291 0008-4042 |
| DOI: | 10.1139/cjc-2018-0258 |
| Popis: | A simple and selective colorimetric method for detection of cysteine using chitosan stabilized gold nanoparticles has been developed. L-cysteine is a sulfur containing amino acid. Cysteine binds with the gold nanoparticles through a sulfur group and induces aggregation of the nanoparticles, which leads to a visible colour change from pale red to blue. A good linearity was observed from 0.1 to 30 μmol/L (R = 0.9958) cysteine, and the limit of detection was found to be 0.1 μmol/L. The gold nanoparticles showed a high selectivity towards the detection of cysteine even in the presence of 10 000 fold higher concentration of common interferences such as glutathione, methionine, and homocysteine. A plausible mechanism for the selective detection of cysteine in presence of interferences based on a dispersion–aggregation mechanism has been proposed. |
| Databáze: | OpenAIRE |
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