Using Elisa to evaluate complement activation by reference biomaterials

Autor:	M. Berger, B. Broxup, M. V. Sefton
Rok vydání:	1994
Předmět:	Materials science Biomedical Engineering Biophysics Biomaterial Bioengineering Cellulose acetate In vitro Complement (complexity) Dialysis tubing Complement system Biomaterials chemistry.chemical_compound Adsorption chemistry iC3b Organic chemistry
Zdroj:	Journal of Materials Science: Materials in Medicine. 5:622-627
ISSN:	1573-4838 0957-4530
DOI:	10.1007/bf00120343
Popis:	The interaction between blood and an artificial surface results in the activation of complement and can lead to extensive inflammatory reactions. In vitro work was undertaken to validate a system of assays to evaluate the complement activation of a candidate biomaterial by comparing its capacity of activation with that of reference biomaterials. The biomaterials tested were cellulose acetate, cellulose dialysis tubing, low density polyethylene, polydimethylsiloxane and AN69. Complement activation was assessed using ELISA for iC3b, C3a and Factor Bb. Results showed that (1) iC3b measurements may underestimate complement activation, (2) the presence of Bb in plasma or serum was a reliable indicator of complement activation, (3) measurements of C3a are of interest but are difficult, (4) adsorption or/and binding of complement proteins occured on the surface. Evaluation of complement activation for a biomaterial should include the assessment of the fluid phase (in serum or plasma) as well as the bound phase (on the surface of the biomaterial) of complement.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8270f8832d179eb2b64890d38b9402fb https://doi.org/10.1007/bf00120343 Zobrazit plný text záznamu Full text from SpringerLink