Oxygen-Uptake and Mass-Transfer Rates on the Growth of Pseudomonas putida CECT5279: Influence on Biodesulfurization (BDS) Capability
| Autor: | A. Alcon, Victoria E. Santos, Felix Garcia-Ochoa, and Ana B. Martin, Emilio Miraflores Gómez |
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| Rok vydání: | 2006 |
| Předmět: |
biology
Chemistry General Chemical Engineering Analytical chemistry Energy Engineering and Power Technology chemistry.chemical_element Kinetic energy biology.organism_classification Oxygen uptake Oxygen Pseudomonas putida chemistry.chemical_compound Fuel Technology Dibenzothiophene Mass transfer Yield (chemistry) Bioreactor |
| Zdroj: | Energy & Fuels. 20:1565-1571 |
| ISSN: | 1520-5029 0887-0624 |
| DOI: | 10.1021/ef050362y |
| Popis: | Oxygen-uptake and oxygen mass-transfer rates in cultures of Pseudomonas putida [a genetically modified organism (GMO) able to desulfurize dibenzothiophene and its derivatives] have been determined in a stirred tank bioreactor under different transport conditions. The oxygen-uptake rate has been measured by applying a modified dynamic method using a step of pure oxygen, because of the very low oxygen concentration in the broth. A kinetic model is proposed, obtaining the kinetic parameter values: specific maintenance and yield coefficients. Volumetric mass-transfer coefficient, kLa, has been determined, in a wide interval of hydrodynamic conditions, and predicted using a theoretical model. An increase of the transport rate is detected with the increase of the biomass and oxygen-uptake rate, which is described by a biological enhancement factor, E, which is also calculated by a theoretical model. Experimental values of kLa and E are compared with those predicted by the models with good agreement. Influences... |
| Databáze: | OpenAIRE |
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